Publication List



An optogenetic assay method for electrogenic transporters using Escherichia coli co-expressing light-driven proton pump. Hayashi M,  Kojima K, Sudo Y*, Yamashita A.* Protein. Sci., 30, 2161-2169, 2021.   PubMed

(Highlighted in “In This Issue.”   Link )

A sweet protein monellin as a non-antibody scaffold for synthetic binding proteins. Yasui N*Nakamura K, Yamashita A. J. Biochem., 169, 585-599.   PubMed

Structural studies of reelin N-terminal region provides insights into a unique structural arrangement and functional multimerization. Nagae M, Suzuki K, Yasui N, Nogi T, Kohno T, Hattori M, Takagi J.* J. Biochem., 169, 555-564.   PubMed


Current pivotal strategies leading a difficult target protein to a sample suitable for crystallographic analysis. Yamashita A.* Biochem. Soc. Trans., 48, 1661-1673, 2020.   PubMed   <Review>


Differential scanning fluorimetric analysis of the amino-acid binding to taste receptor using a model receptor protein, the ligand-binding domain of fish T1r2a/T1r3. Yoshida T, Yasui N, Kusakabe Y, Ito C, Akamatsu M, Yamashita A.* PLoS ONE, 14, e0218909, 2019.      Link

Specific modification at the C-terminal lysine residue of the green fluorescent protein variant, GFPuv, expressed in Escherichia coli. Nakatani T, Yasui N*, Tamura I, Yamashita A.* Sci. Rep., 9, 4722, 2019. (co-first authors)   PubMed


Structure-function relationships of olfactory and taste receptors. Behrens M, Briand L, de March CA, Matsunami H, Yamashita A, Meyerhof W, Weyand S.* Chem. Senses., 43, 81-87, 2018.   PubMed   <Review>


A large-scale expression strategy for multimeric extracellular protein complexes using Drosophila S2 cells and its application to the recombinant expression of heterodimeric ligand-binding domains of taste receptor. Yamashita A.*, Nango E, Ashikawa Y. Prot. Sci., 26, 2291-2301, 2017.   PubMed

Structural basis for ligand capture and release by the endocytic receptor ApoER2. Hirai H, Yasui N, Yamashita K, Tabata S, Yamamoto M, Takagi J, Nogi T.* EMBO Rep., 8, 982-999, 2017. (co-first authors)   PubMed

Structural basis for perception of diverse chemical substances by T1r taste receptors. Nuemket N, Yasui N, Kusakabe Y, Nomura Y, Atsumi N, Akiyama S, Nango E, Kato Y, Kaneko MK, Takagi J, Hosotani M, Yamashita A.* Nat Commun., 8,15530, 2017. (co-first authors)   PubMed

Immunoaffinity Purification of the Glycosylated Extracellular Fragment of Mouse Plexin A2 Produced in a Mammalian Expression System. Nogi T.*, Mihara E., Yasui N., Takagi J. Methods Mol Biol. 1493, 57-72, 2017.   PubMed   <Book chapter>


Taste substance binding elicits conformational change of taste receptor T1r heterodimer extracellular domains. Nango E, Akiyama S, Maki-Yonekura S, Ashikawa Y, Kusakabe Y, Krayukhina E, Maruno T, Uchiyama S, Nuemket N, Yonekura K, Shimizu M, Atsumi N, Yasui N, Hikima T, Yamamoto M, Kobayashi Y, Yamashita A.* Sci. Rep., 6, 25745, 2016. PubMed 


Studies on an acetylcholine binding protein identify a basic residue on the β1-strand as a new structural determinant of neonicotinoid actions. Ihara M, Okajima T, Yamashita A, Oda T, Asano T, Matsui M, Sattelle DB, Matsuda K.* Mol. Pharmacol., 86, 736-746, 2014. (co-first authors)   PubMed

Distinct human and mouse membrane trafficking systems for sweet taste 1 receptors T1r2 and T1r3. Shimizu M, Goto M, Kawai T, Yamashita A, Kusakabe, Y.* PLoS ONE 9, e100425, 2014.   PubMed

General flexible nature of the cytosolic regions of fungal TRP channels, revealed by expression screening using GFP-fusion techniques. Ihara M, Takano Y, Yamashita A.* Prot. Sci. 23,923-931, 2014.   PubMed

Directed network wiring identifies a key protein interaction in embryonic stem cell differentiation. Yasui N, Findlay GM, Gish GD, Hsiung MS, Huang J, Tucholska M, Taylor L, Smith L, Boldridge WC, Koide A, Pawson T, Koide S.* Mol Cell. 54, 1034-41, 2014.   PubMed


Molecular bases of multimodal regulation of a fungal transient receptor potential (TRP) channel. Ihara M, Hamamoto S, Miyanoiri Y, Takeda M, Kainosho M, Yabe I, Uozumi N, Yamashita A.* J Biol Chem. 288, 15303–17, 2013.   PubMed

Publication in JapaneseJpn._review.html