(2024)

Sweet/umami taste receptor-mediated chloride-sensation revealed by X-ray crystallographic analysis. Yamashita A. SPring-8/SACLA Research Frontiers 2023, 36-37, 2024.   Link   <research highlight>

Unravelling nicotinic receptor and ligand features underlying neonicotinoid knockdown actions on the malaria vector mosquito Anopheles gambiae. Ito R, Kamiya M, Takayama K, Mori S, Matsumoto R, Takebayashi M, Ojima H, Fujimura S, Yamamoto H, Ohno M, Ihara M, Okajima T, Yamashita A, Colman F, Lycett GJ, Sattelle DB, Matsuda K.* Open Biol. 14, 240057, 2024.   PubMed

Overexpression of the flagellar motor protein MotB sensitizes Bacillus subtilis to aminoglycosides in a motility-independent manner. Uneme M, Ishikawa K, Furuta K, Yamashita A, Kaito C*  PLoS One 19, e0300634, 2024.   PubMed

Chemical range recognized by the ligand-binding domain in a representative amino acid-sensing taste receptor, T1r2a/T1r3, from medaka fish. Ishida H, Yasui N, Yamashita A.*  PLoS One 19, e0300981, 2024.   PubMed

Accelerated Molecular Dynamics and AlphaFold Uncover a Missing Conformational State of Transporter Protein OxlT. Ohnuki J, Jaunet-Lahary T, Yamashita A, Okazaki KI.*  J Phys Chem Lett. 15, 725-732, 2024.   PubMed

(2023)

Investigating the Effect of Substituting a Single Cysteine Residue on the Thermal Stability of an Engineered Sweet Protein, Single-Chain Monellin. Ohnuma K, Yamashita A, Yasui N*.  Protein J. 42, 698-708, 2023.   PubMed

Direct binding of calmodulin to the cytosolic C-terminal regions of sweet/umami taste receptors. Yoshida A, Ito A, Yasui N*, Yamashita A.* J. Biochem. 174, 451-459, 2023.   PubMed

Structure of the thrombopoietin-MPL receptor complex is a blueprint for biasing hematopoiesis. Tsutsumi N*, Masoumi Z, James SC, Tucker JA, Winkelmann H, Grey W, Picton LK, Moss L, Wilson SC, Caveney NA, Jude KM, Gati C, Piehler J, Hitchcock IS*, Garcia KC.* Cell, 186, 4189-4203.e22, 2023.   PubMed

Structural insight into guanylyl cyclase receptor hijacking of the kinase-Hsp90 regulatory mechanism. Caveney NA*, Tsutsumi N, Garcia KC.* eLife, 12, RP86784, 2023.   PubMed

Structure and mechanism of oxalate transporter OxlT in an oxalate-degrading bacterium in the gut microbiota. Jaunet-Lahary T, Shimamura T*, Hayashi M, Nomura N, Hirasawa K, Shimizu T, Yamashita M, Tsutsumi N, Suehiro Y, Kojima K, Sudo Y, Tamura T, Iwanari H, Hamakubo T, Iwata S, Okazaki KI*, Hirai T*, Yamashita A.* Nat Commun. 14, 1730, 2023.   PubMed

Structural basis of Janus kinase trans-activation. Caveney NA, Saxton RA, Waghray D, Glassman CR, Tsutsumi N, Hubbard SR, Garcia KC.* Cell Rep. 42, 112201, 2023.  PubMed

Structure of the Wnt-Frizzled-LRP6 initiation complex reveals the basis for coreceptor discrimination. Tsutsumi N, Hwang S, Waghray D, Hansen S, Jude KM, Wang N, Miao Y, Glassman CR, Caveney NA, Janda CY, Hannoush RN, Garcia KC.* Proc Natl Acad Sci U S A. 120, e2218238120, 2023.  PubMed

Chloride ions evoke taste sensations by binding to the extracellular ligand-binding domain of sweet/umami taste receptors. Atsumi N†, Yasumatsu K†, Takashina Y, Ito C, Yasui N, Margolskee RF, Yamashita A.* Elife, 12, e84291, 2023. (†co-first authors) PubMed

Pivotal role for S-nitrosylation of DNA methyltransferase 3B in epigenetic regulation of tumorigenesis. Okuda K, Nakahara K, Ito A, Iijima Y, Nomura R, Kumar A, Fujikawa K, Adachi K, Shimada Y, Fujio S, Yamamoto R, Takasugi N, Onuma K, Osaki M, Okada F, Ukegawa T, Takeuchi Y, Yasui N, Yamashita A, Marusawa H, Matsushita Y, Katagiri T, Shibata T, Uchida K, Niu SY, Lang NB, Nakamura T, Zhang KYJ, Lipton SA, Uehara T.* Nat Commun. 14, 621, 2023.  PubMed

(2022)

Development of hidden Markov modeling method for molecular orientations and structure estimation from high-speed atomic force microscopy time-series images. Ogane T, Noshiro D, Ando T, Yamashita A, Sugita Y, Matsunaga Y* PLoS Comput Biol.,18, e1010384, 2022.  PubMed

T1R2-mediated sweet sensing in a lizard. Liang Q, Ko MC, Ng NSR, Reh B, Lee JGH, Yamashita A, Nishihara H, Toda Y, Baldwin MW.* Curr Biol., 32, R1302-R1303, 2022.  PubMed

Viral G Protein-Coupled Receptors Encoded by β- and γ-Herpesviruses. Rosenkilde MM, Tsutsumi N, Knerr JM, Kildedal DF, Garcia KC.* Annu Rev Virol., 9, 329-351, 2022.  PubMed   <Review>

Organizing structural principles of the IL-17 ligand-receptor axis. Wilson SC, Caveney NA, Yen M, Pollmann C, Xiang X, Jude KM, Hafer M, Tsutsumi N, Piehler J, Garcia KC.* Nature, 609, 622-629, 2022.  PubMed

Structure of the IL-27 quaternary receptor signaling complex. Caveney NA, Glassman CR, Jude KM, Tsutsumi N, Garcia KC.* eLife, 11, e78463, 2022.  PubMed

Structure of a Janus kinase cytokine receptor complex reveals the basis for dimeric activation. Glassman CR†, Tsutsumi N†, Saxton RA, Lupardus PJ, Jude KM, Garcia KC.* Science, 376, 163-169, 2022. (†co-first authors)   PubMed

(2021)

An optogenetic assay method for electrogenic transporters using Escherichia coli co-expressing light-driven proton pump. Hayashi M,  Kojima K, Sudo Y*, Yamashita A.* Protein. Sci., 30, 2161-2169, 2021.   PubMed

(Highlighted in “In This Issue.”   Link )

A sweet protein monellin as a non-antibody scaffold for synthetic binding proteins. Yasui N*,  Nakamura K, Yamashita A. J. Biochem., 169, 585-599.   PubMed

Structural studies of reelin N-terminal region provides insights into a unique structural arrangement and functional multimerization. Nagae M, Suzuki K, Yasui N, Nogi T, Kohno T, Hattori M, Takagi J.* J. Biochem., 169, 555-564.   PubMed

(2020)

Current pivotal strategies leading a difficult target protein to a sample suitable for crystallographic analysis. Yamashita A.* Biochem. Soc. Trans., 48, 1661-1673, 2020.   PubMed   <Review>

(2019)

Differential scanning fluorimetric analysis of the amino-acid binding to taste receptor using a model receptor protein, the ligand-binding domain of fish T1r2a/T1r3. Yoshida T, Yasui N, Kusakabe Y, Ito C, Akamatsu M, Yamashita A.* PLoS ONE, 14, e0218909, 2019.      Link

Specific modification at the C-terminal lysine residue of the green fluorescent protein variant, GFPuv, expressed in Escherichia coli. Nakatani T†, Yasui N†*, Tamura I, Yamashita A.* Sci. Rep., 9, 4722, 2019. (†co-first authors)   PubMed

(2018)

Structure-function relationships of olfactory and taste receptors. Behrens M, Briand L, de March CA, Matsunami H, Yamashita A, Meyerhof W, Weyand S.* Chem. Senses., 43, 81-87, 2018.   PubMed   <Review>

(2017)

A large-scale expression strategy for multimeric extracellular protein complexes using Drosophila S2 cells and its application to the recombinant expression of heterodimeric ligand-binding domains of taste receptor. Yamashita A.*, Nango E, Ashikawa Y. Prot. Sci., 26, 2291-2301, 2017.   PubMed

Structural basis for ligand capture and release by the endocytic receptor ApoER2. Hirai H†, Yasui N†, Yamashita K, Tabata S, Yamamoto M, Takagi J, Nogi T.* EMBO Rep., 8, 982-999, 2017. (†co-first authors)   PubMed

Structural basis for perception of diverse chemical substances by T1r taste receptors. Nuemket N†, Yasui N†, Kusakabe Y, Nomura Y, Atsumi N, Akiyama S, Nango E, Kato Y, Kaneko MK, Takagi J, Hosotani M, Yamashita A.* Nat Commun., 8,15530, 2017. (†co-first authors)   PubMed

Immunoaffinity Purification of the Glycosylated Extracellular Fragment of Mouse Plexin A2 Produced in a Mammalian Expression System. Nogi T.*, Mihara E., Yasui N., Takagi J. Methods Mol Biol. 1493, 57-72, 2017.   PubMed   <Book chapter>

(2016)

Taste substance binding elicits conformational change of taste receptor T1r heterodimer extracellular domains. Nango E, Akiyama S, Maki-Yonekura S, Ashikawa Y, Kusakabe Y, Krayukhina E, Maruno T, Uchiyama S, Nuemket N, Yonekura K, Shimizu M, Atsumi N, Yasui N, Hikima T, Yamamoto M, Kobayashi Y, Yamashita A.* Sci. Rep., 6, 25745, 2016. PubMed 

(2014)

Studies on an acetylcholine binding protein identify a basic residue on the β1-strand as a new structural determinant of neonicotinoid actions. Ihara M†, Okajima T†, Yamashita A†, Oda T, Asano T, Matsui M, Sattelle DB, Matsuda K.* Mol. Pharmacol., 86, 736-746, 2014. (†co-first authors)   PubMed

Distinct human and mouse membrane trafficking systems for sweet taste 1 receptors T1r2 and T1r3. Shimizu M, Goto M, Kawai T, Yamashita A, Kusakabe, Y.* PLoS ONE 9, e100425, 2014.   PubMed

General flexible nature of the cytosolic regions of fungal TRP channels, revealed by expression screening using GFP-fusion techniques. Ihara M, Takano Y, Yamashita A.* Prot. Sci. 23,923-931, 2014.   PubMed

Directed network wiring identifies a key protein interaction in embryonic stem cell differentiation. Yasui N, Findlay GM, Gish GD, Hsiung MS, Huang J, Tucholska M, Taylor L, Smith L, Boldridge WC, Koide A, Pawson T, Koide S.* Mol Cell. 54, 1034-41, 2014.   PubMed

(2013)

Molecular bases of multimodal regulation of a fungal transient receptor potential (TRP) channel. Ihara M, Hamamoto S, Miyanoiri Y, Takeda M, Kainosho M, Yabe I, Uozumi N, Yamashita A.* J Biol Chem. 288, 15303–17, 2013.   PubMed