小胞型ポリアミントランスポーター

Vesicular Polyamine Transporter

小胞型ポリアミントランスポーターの発見と機能

1.       日浅 未来 (2018) Polyamineのマスト細胞分泌顆粒内蓄積と分泌機構　臨床免疫・アレルギー科　69, 257-263

2.       Takeuchi, T., Harada, Y., Moriyama, S., Furuta, K., Tanaka, S., Miyaji, T., Omote, H., Moriyama. Y., and Hiasa, M. (2017) Vesicular polyamine transporter mediates vesicular storage and release of polyamine from mast cells. J. Bio.l Chem. 292, 3909-3918. 

3.       Hiasa, M., Miyaji, T., Haruna, Y., Takeuchi, T., Harada, Y., Moriyama, S., Yamamoto, A., *Omote, H., Moriyama, Y. (2014) Identification of a mammalian vesicular polyamine transporter.  Scientific Reports 4, 6836. 

小胞型ヌクレオチドトランスポーター

Vesicular Nucleotide Transporter

小胞型ヌクレオチドトランスポーターの発見と機能　

1.       Tatsushima K., Hasuzawa N., Wang L., Hiasa M., Sakamoto S., Ashida K., Sudo N., Moriyama Y., and Nomura M. (2020) Vesicular ATP release from hepatocytes plays a role in the progression of nonalcoholic steatohepatitis. Biochim Biophys Acta Mol Basis Dis. 166013.

2.       Iwai, Y., Kamatani, S., Moriyama, S., and *Omote, H. (2019) Function of essential chloride and arginine residue in nucleotide binding to vesicular nucleotide transporter. J. Biochem. 165, 479-486.

3.       Kinoshita M., Hirayama Y., Fujishita K., Shibata K., Shinozaki Y., Shigetomi E., Takeda A., Le HPN, Hayashi H., Hiasa M., Moriyama Y., Ikenaka K., Tanaka KF., and Koizumi S. (2018) Anti-Depressant Fluoxetine Reveals its Therapeutic Effect Via Astrocytes. EBioMedicine. 32, 72-83.

4.       Harada Y., Kato Y., Miyaji T., Omote, H., Moriyama Y., and *Hiasa M. (2018) Vesicular nucleotide transporter mediates ATP release and migration in neutrophils.  J. Bio.l Chem. 293, 3770-3779.

5.       Kato Y., Hiasa M., Ichikawa R., Hasuzawa N., Kadowaki A., Iwatsuki K., Shima K., Endo Y., Kitahara Y., Inoue T., Nomura M., Omote H., Moriyama Y., and Miyaji T. (2017) Identification of a vesicular ATP release inhibitor for the treatment of neuropathic and inflammatory pain. Proc Natl Acad Sci U S A. 114, E6297-E6305.

6.       Moriyama Y., Hiasa M., Sakamoto S., Omote H., and Nomura M. (2017) Vesicular nucleotide transporter (VNUT): appearance of an actress on the stage of purinergic signaling. Purinergic Signal. 13, 387-404.

7.       Nakagomi H,. Yoshiyama M,. Mochizuki T., Miyamoto T., Komatsu R., Imura Y., Morizawa Y., Hiasa M., Miyaji T., Kira S., Araki I., Fujishita K., Shibata K., Shigetomi E., Shinozaki Y., Ichikawa R., Uneyama H., Iwatsuki K., Nomura M., de Groat WC., Moriyama Y., Takeda M., and Koizumi S. (2016) Urothelial ATP exocytosis: regulation of bladder compliance in the urine storage phase. Sci Rep., 6, 29761.

8.       Moriyama S., and *Hiasa M. (2016) Expression of Vesicular Nucleotide Transporter in the Mouse Retina. Biol Pharm Bull. 39, 564-569.

9.       Sakamoto,S., Miyaji, T., Hiasa, M., Ichikawa, R., Uematsu, A., Iwatsuki, K., Shibata,A.,  Uneyama, H., Takayanagi, R., Yamamoto, A.,Omote, H., *Nomura, M., *Moriyama, Y. (2014) Impairment of vesicular ATP release affects glucose metabolism and increases insulin sensitivity. Scientific Reports 4, 6689.

10.    Hiasa M., Togawa N., and Moriyama Y. (2014) Vesicular Nucleotide Transport: a Brief History and the Vesicular Nucleotide Transporter as a Target for Drug Development. Curr Pharm Des., 20, 2745-2749.

11.    Hiasa, M., Togawa, N., Miyaji, T., Omote, H. Yamamoto, A., *Moriyama, Y. (2014) Essential role of vesicular nucleotide transporter in vesicular storage and release of nucleotides in platelets.Physiol Rep. 2, pii. e12034.

12.    Harada Y., and *Hiasa M. (2014) Immunological identification of vesicular nucleotide transporter in intestinal L cells. Biol Pharm Bull. 37, 1090-1095

13.    Sesma JI., Kreda SM., Okada SF., van Heusden C., Moussa L., Jones LC., O'Neal WK., Togawa N., Hiasa M., Moriyama Y., and Lazarowski ER. (2013) The vesicular nucleotide transporter (VNUT) regulates the nucleotide content in airway epithelial mucin granules. Am J Physiol Cell Physiol. 304, C976-984.

14.    Kato,Y., Omote, H., Miyaji, T. (2013) Inhibitors of ATP release inhibit vesicular nucleotide transporter. Biol. Pharm. Bull. 36, 1688-1691.　

15.    Larsson M., Sawada K., Morland C., Hiasa M., Ormel L., Moriyama Y., and Gundersen V. (2012) Functional and anatomical identification of a vesicular transporter mediating neuronal ATP release. Cereb Cortex. 22, 1203-1214

16.    Moriyama, Y., Omote, H., Sawada, K. 2012 Sep 26, Novel SLC-17-type transporter protein in mammal and use thereof. European Patent EP 2159281B1

17.    Moriyama, Y., Omote, H., Sawada, K. 2011 May 31, Methods for screening modulators of SLC17-type anion transport activity, United States Patent US7,951,595B2

18.    Miyaji, T., Sawada, K., Omote, H., *Moriyama, Y. (2011) Divalent cation transport by vesicular nucleotide transporter.  J. Biol. Chem. 286, 42881-42887.

19.    Iwatsuki K., Ichikawa R., Hiasa M., Moriyama Y., Torii K., and Uneyama H. (2009) Identification of the vesicular nucleotide transporter (VNUT) in taste cells. Biochem Biophys Res Commun. 388, 1-5.

20.    Sawada, K., Echigo, N., Juge, N., Miyaji, T., Otsuka, M., Omote, H., Yamamoto, A. and *Moriyama, Y.  (2008)  Identification of a vesicular nucleotide transporter.  Proc. Natl. Acad. Sci. USA. 105, 5683-5686.

小胞型グルタミン酸、アスパラギン酸、GABAトランスポーター

Vesicular Glutamate, Aspartate, GABA Transporter

小胞型神経伝達物質トランスポーターの構造と機能

1.       Matsuo, S., *Hiasa, M., and *Omote, H. (2020) Functional characterization and tissue localization of the facilitative glucose transporter GLUT12. J. Biochem. doi: 10.1093/jb/mvaa090.

2.       *Omote, H., and Moriyama Y.  (2018) Reconstitution and transport analysis of eukaryotic transporters in the post-genomic era.  Methods. Mol. Biol. 1700, 343-352. 

3.       Moriyama S., Iharada M., Omote H., Moriyama Y., and Hiasa M. (2017) Function and expression of a splicing variant of vesicular glutamate transporter 1. Biochimica et Biophysica Acta (BBA) – Biomembranes, 1859, 931-940.

4.       *Omote, H., Miyaji, T., Hiasa, M., Juge, N., ^*Moriyama, Y. (2016) Structure, function, and drug interactions of neurotransmitter transporters in the post-genome era. Annu. Rev. Pharmacol. Toxicol. 56, 385-402.

5.       Hara C., Kubota S., Nishida T., Hiasa M., Hattori T., Aoyama E., Moriyama Y., Kamioka H., and Takigawa M. (2016) Involvement of multiple CCN family members in platelets that support regeneration of joint tissues. Mod Rheumatol. 26, 940-949.

6.       Miyaji, T., Kuromori, T., Takeuchi, Y., Yamaji, N., Yokosho, K., Shimazawa, A., Sugimoto, E., Omote, H., Ma, JF., Shinozaki, K., Moriyama, Y. (2015) AtPHT4;4 is a chloroplast-localized ascorbate transporter in Arabidopsis. Nat. Commun. 6, 5928.

7.       Juge N., Omote, H., *Moriyama Y. (2013) Vesicular GABA transporter (VGAT) transports β-alanine. .J Neurochem. 127, 482-486.　

8.       Omote, H., *Moriyama, Y. (2013) Vesicular Neurotransmitter Transporters: An approach for studying transporters with purified proteins.  Physiology 28, 39-50.

9.       表　弘志、森山芳則　(2013)　塩素イオンにより制御されるトランスポーター群の発見と意義　医学の歩み　245, 69-73.

10.    *Omote, H., Miyaji, T., Juge, N. and Moriyama, Y. (2011) Vesicular neurotransmitter transporter: bioenergetics and regulation of glutamate transport.  Biochemistry 50, 5558-5565.

11.    Miyaji, T., Omote, H., *Moriyama, Y. (2011) Functional characterization of vesicular excitatory amino acid transport by human sialin. 
J. Neurochem. 119, 1-5.

12.    Juge, N., Gray, J.A., Omote, H., Miyaji, T., Inoue, T., Hara, C., Uneyama, H., Edwards, R.H., Nicoll, R.A. and *Moriyama, Y. (2010) Metabolic control of vesicular glutamate transport and release.    Neuron 68, 99-112.   

13.    Sonoda, Y., Cameron, A., Newstead, S., Omote, H., Moriyama, Y., Kasahara, M., *Iwata, S., *Drew, D. (2010) Tricks of the trade used to accelerate high-resolution structure determination of membrane proteins. FEBS 584, 2539-2547.

14.   Juge, N., Muroyama, A., Hiasa, M., Omote, H. and *Moriyama, Y.  (2009)  Vesicular inhibitory amino acid transporter is a Cl^-/γ-aminobutyrate co-transporter.  J. Biol. Chem. 284, 35073-35078. FEBS 584, 2539-257.

15.    Miyaji, T., Echigo, N., Hiasa, M., Senoh, S., Omote, H. and *Moriyama, Y.  (2008)  Identification of a vesicular aspartate transporter.     Proc. Natl. Acad. Sci. USA. 105, 11720-11724.

16.   森山芳則、表　弘志、宮地孝明、日浅未来　平成25年7月19日「アスパラギン酸の化学伝達を司るトランスポーターの同定とその利用」特許第5317056号

17.    森山芳則、表弘志、樹下成信　平成20年3月18日　興奮性化学伝達調節剤およびそのスクリーニング法　特許第503889号

18.   *表弘志、樹下成信 (2007) 小胞型グルタミン酸トランスポーターの分子機構 生化学　79, 956-960.

19.   Iharada M., Hiasa M., Kobara A., and Moriyama Y. (2007) Exocytosis of D-aspartate from INS-1E clonal beta cells. Biol. Pharm. Bull., 30, 1329-1331.

20.   Hiasa M., and Moriyama Y. (2006) Immunohistochemical localization of D-aspartate in islets of Langerhans. Biol. Pharm. Bull., 29, 1251-1253.

21.   Juge, N., Yoshida, Y., Yatsushiro, S., *Omote, H., Moriyama, Y. (2006) Vesicular glutamate transporter contains two independent transport machineries  J.Biol.Chem. 281, 39499-39506.

22.   Morimoto, R., Uehara, S., Yatsushiro, S., Juge, N., Hua, Z., Senoh, S., Echigo, N., Hayashi, M., Mizoguchi, T., Ninomiya, T., Udagawa, N., Omote, H., Yamamoto, A., Edwards, R.H. and *Moriyama, Y. (2006) Secretion of L-glutamate from osteoclasts through transcytosis.  EMBO J. 25, 4175-4186.

23.   Uehara S., Jung SK., Morimoto R., Arioka S., Miyaji T., Juge N., Hiasa M., Shimizu K., Ishimura A., Otsuka M., Yamamoto A., Maechler P., and Moriyama Y. (2006) Vesicular storage and secretion of L-glutamate from glucagons-like peptide 1-secreting　clonal intestinal L cells. J. Neurochem., 96, 550-560.

24.   Jung, S.K., Morimoto, R., Otsuka, M. and *Omote, H. (2005) Transmembrane topology of vesicular glutamate transporter 2.  Biol. Pharm. Bull. 29, 547-549.

マラリア原虫、バクテリアおよびヒトの薬物排出トランスポーターの輸送機能解析

Drug Transporters

Functional characterization of drug transporters

1.       Nagarathinam, K., Nakada-Nakura, Y., Parthier, C., Terada, T., Juge, N., Jaenecke, F., Liu, K., Hotta, Y., Miyaji T., Omote, H, Iwata, S., Nomura, N., Stubbs, M.T., and Tanabe, M. (2018) Outward open conformation of a Major Facilitator Superfamily multidrug/H⁺ antiporter provides insights into switching mechanism. Nat. Commun. 9, 4005.

2.       Togawa, N., Juge, N., Miyaji, T., Hiasa, M., Omote, H., ^*Moriyama, Y. (2015) Wide expression of type I Na⁺-phosphate cotransporter 3 (NPT3/SLC17A2), a membrane potential-driven organic anion transporter.   Am. J. Physiol. Cell Physiol. 309, C71-80.　

3.       Juge, N., Moriyama, S., Miyaji, T., Kawakami, M., Iwai, H., Fukui, T., Nelson, N., *Omote, H., *Moriyama, Y. (2015) Plasmodium falciparum chloroquine resistance transporter is a H⁺-coupled polyspecific nutrient and drug exporter.   Proc. Natl. Acad. Sci. USA. 112, 3356-3361.  

4.       Miyaji, T., Kawasaki, T., Togawa, N.,Omote, H., *Moriyama, Y. (2013) Type 1 Sodium-Dependent Phosphate Transporter acts as a Membrane Potential-Driven Urate Exporter. Curr Mol Pharmacol. 6, 88-94.

5.       Togawa N, Miyaji T, Izawa S, Omote, H., *Moriyama Y. (2012) A Na⁺-phosphate cotransporter homologue (SLC17A4 protein) is an intestinal organic anion exporter. 
Am J Physiol Cell Physiol. 302, C1652-C1660.

6.       Iharada, M., Miyaji, T., Fujimoto, T., Hiasa, M., Anzai, N., Omote, H., and *Moriyama, Y. (2010) Type 1 sodium dependent phosphate transporter (SLC17A1 protein) is a Cl^--dependent urate exporter.  J. Biol. Chem. 285, 26107-26113.

7.       Ushijima H., Hiasa M., Namba T., Hwang HJ., Hoshino T., Mima S., Tsuchiya T., Moriyama Y., and Mizushima T. (2008) Expression and function of TETRAN, a new type of membrane transporter. Biochem. Biophys. Res. Commun., 374, 325-330.

8.       Omote, H., *Al-Shawi, MK., (2006) Interaction of transported drugs with the lipid bilayer and P-glycoprotein through a solvation exchange mechanism. Biophys. J. 90, 4046-4059.

9.       Ohashi-Kobayashi, A., Ohashi, K., Du, WB., Omote, H., Nakamoto, RK., Al-Shawi, MK., *Maeda, M. (2006) Examination of drug resistance activity of human TAP-like (ABCB9) expressed in yeast. Biochem. Biophys. Res. Commun. 343, 597-601.

10.    *Al-Shawi, MK., Omote, H. (2005) The remarkable transport mechanism of P-glycoprotein: a multidrug transporter.  J. Bioenerg. Biomembr. 37, 489-496.

11.    Otsuka, M., Yasuda, M., Morita, Y., Otsuka, C., Tsuchiya, T., Omote, H., *Moriyama, Y.  (2005) Identification of essential amino acid residues of the NorM Na⁺/multidrug antiporter in Vibrio parahaemolyticus. J. Bacteriol. 187, 1552-1558.

12.    Omote, H., Polar, M.K., Figler, R.A. and *Al-Shawi, M.K  (2004) Improved Energy Coupling of Human P-glycoprotein by the Glycine 185 to Valine Mutation.  Biochemistry 43, 3917-3928.

13.    Al-Shawi, M.K., Polar, M.K., Omote, H., Figler, R.A.  (2003) Transition state analysis of the coupling of drug transport to ATP hydrolysis by P-glycoprotein.  J. Biol. Chem. 278, 52629-52640.

14.    Omote, H., and Al-Shawi, M. K. (2002) A novel electron paramagnetic resonance approach to determine the mechanism of drug transport by P-glycoprotein.   J. Biol. Chem. 277, 45688-45694.

15.    Figler, R. A., Omote, H., Nakamoto, R. K., and Al-Shawi, M. K. (2000) Use of chemical chaperones in the yeast Saccharomyces cerevisiae to enhance heterologous membrane protein expression: high-yield expression and purification of human P-glycoprotein.  Arch. Biochem. Biophys. 376, 34-46.

MATE型薬物排出トランスポーターの発見と機能解析

MATE Type Drug Transporters

Identification and functional characterization of MATE type drug transporters

1.       Kawasaki, T., Matsumoto, T., Iwai, Y., Kawakami, M., Juge, N., *Omote, H., Nabekura, T., and Moriyama, Y. (2018) Purification and reconstitution of polyspecific H⁺/organic cation antiporter human MATE1.  Biochim. Biophys. Acta Biomembr.  1860, 2456-2464.

2.       Shitan, N., Minami, S., Morita, M., Hayashida, M., Ito, S., Takanashi, K., Omote, H., Moriyama, Y., Sugiyama, A., Goossens, A., Moriyasu, M., *Yazaki, K. (2014) Involvement of the leaf-specific multidrug and toxic compound extrusion (MATE) transporter Nt-JAT2 in vacuolar sequestration of nicotine in Nicotiana tabacum.　 Plos One 9(9):e108789.

3.       Kawasaki, T., Ito, H., *Omote, H. (2014) Components of foods inhibit a drug exporter, human multidrug and toxin extrusion transporter. Biol. Pharm. Bull. 37, 292-297.

4.       Komatsu, T., Hiasa, M., Miyaji, T., Kanamoto, T., Matsumoto, T., Otsuka, M., Moriyama, Y. and　*Omote, H (2011) Characterization of the human MATE2 proton-coupled　polyspecific organic cation exporter. J. Biochem. Cell Biol. 43, 913-918.

5.       Moriyama, Y., Omote, H., Otsuka, M., Matsumoto, T., Hiasa, M.. 2010, Nov. 4, Novel Transporter protein in mammal and utilization of the same.  United States Patent US7,892,728B2　

6.       森山芳則、表  弘志、大塚正人、松本拓也、日浅未来 平成26年8月8日「哺乳動物における新規トランスポータータンパク質およびその利用」特許5590590号　

7.       森山芳則、表　弘志、大塚正人、松本拓也、日浅未来　平成23年6月3日　哺乳動物における新規トランスポータータンパク質およびその利用　特許第4752068号

8.       森山芳則、表　弘志、宮地孝明、日浅未来  平成25年1月11日
哺乳動物における新規トランスポータータンパク質およびその利用」 特許第5167547号

9.       Matsumoto, T., Kanamoto, T., Otsuka, M., Omote, H. and *Moriyama, Y.  (2008)  Role of glutamate residues in substrate recognition by human MATE1 polyspecific H⁺/organic cation exporter.  Am. J. Physiol. Cell Physiol. 294, C1074-C1078.

10.    Kobara, A., Hiasa, M., Matsumoto, T., Otsuka, M., Omote, H. and *Moriyama, Y. (2008)  A novel variant of mouse MATE-1 H⁺/organic cation antiporter with a long hydrophobic tail.   Arch. Biochem. Biophys. 469, 195-199.

11.    *Moriyama, Y., Hiasa, M., Matsumoto, T., Omote, H. (2008) Multidrug and toxic compound extrusion (MATE)-type proteins as anchor transporters for the excretion of metabolic waste products and xenobiotics.  Xenobiotica 38, 1107-1118.

12.    薬物排出のアンカートランスポーター、MATEの構造と機能 (2008) 日浅未来、表弘志、*森山芳則　蛋白質核酸酵素53, 52-58.

13.    Hiasa M., Matsumoto T., Komatsu T., Omote H., *Moriyama Y.  (2007) Functional characterization of testis-specific rodent multidrug and toxic compound extrusion 2, a class III MATE-type polyspecific H⁺/organic cation exporter.  Am J Physiol Cell Physiol. 293, C1437-C1444.

14.   Omote, H., Hiasa, M., Matsumoto, T., Otsuka, M. and *Moriyama, Y. (2006) The MATE proteins as fundamental transporters for metabolic and xenobiotic organic cations. TRENDS in Pharmacological Sciences, 27, 587-593.

15.    Otsuka, M., Matsumoto, T., Morimoto, R., Arioka, S., Omote, H. and *Moriyama , Y (2005) A human transporter protein that mediates the final excretion step for toxic organic cations. Proc. Natl. Acad. Sci USA 102, 17923-17928.

F1-ATPase その他

F1-ATPase

大腸菌F1-ATPaseの構造と反応機構

1.       Yatsushiro, S., Taniguchi, S., Mitamura, T., Omote, H., *Moriyama, Y. (2005) Proteolipid of vacuolar H⁺-ATPase of Plasmodium falciparum: cDNA cloning, gene organization and complementation of a yeast null mutant.  Biochim. Biophys. Acta. 1717, 89-96.

2.       Futai, M., Omote, H., Sambongi, Y., and Wada, Y., (2000) Synthase (H⁺ ATPase): coupling between catalysis, mechanical work, and proton translocation.  Biochim. Biophys. Acta. 1458, 276-288.

3.       Le, N. P., Omote, H., Wada, Y., Al-Shawi, M. K., Nakamoto, R. K., and Futai, M (2000) Escherichia coli ATP synthase a subunit Arg-376: the catalytic site arginine does not participate in the hydrolysis/synthesis reaction but is required for promotion to the steady state.  Biochemistry 39, 2778-2783.

4.       Hirata, T., Nakamura, N., Omote, H., Wada, Y. and Futai, M. (2000) Regulation and reversibility of vacuolar H⁺-ATPase. J. Biol. Chem. 275, 386-389.

5.       Sambongi, Y., Iko, Y., Tanabe, M., Omote, H., Iwamoto-Kihara, A., Ueda, I., Yanagida, T., Wada, Y. and Futai, M. (1999) Mechanical rotation of the c subunit oligomer in ATP synthase (F₀F₁): direct observation. Science 286, 1722-1724.

6.       Omote, H., Sambonmatsu, N., Saito, K., Sambongi, Y., Iwamoto-Kihara, A., Yanagida, T., Wada, Y., Futai, M., (1999) The γ-subunit rotation and torque generation in F₁-ATPase from wild-type or uncoupled mutant Escherichia coli.  Proc. Natl. Acad. Sci. U S A. 96, 7780-7784.

7.       Omote, H. and Futai, M. (1998) Mutational analysis of F₁F₀ ATPase: catalysis and energy coupling Acta. Physiol. Scand. Suppl. 643, 177-183.

8.       Omote, H., Tainaka, K., Fujie, K., Iwamoto-Kihara, A., Wada, Y., and Futai, M. (1998)  Stability of the Escherichia coli ATP synthase F₀F₁ complex is dependent on interactions between γGln-269 and the β subunit loop βAsp-301-βAsp-305. Arch. Biochem. Biophys. 358, 277-282.

9.       Yoshimizu, T., Omote, H., Wakabayashi, T., Sambongi, Y., Futai, M.  (1998)  Essential Cys-Pro-Cys motif of Caenorhabditis elegans copper transport ATPase.  Biosci. Biotechnol. Biochem.  62, 1258-1260.

10.    Takeyasu, K., Omote, H., Nettikadan, S., Tokumasu, F., Iwamoto-Kihara, A., Futai, M. (1997)  Atomic force microscopy of Escherichia coli F_oF₁-ATPase in reconstituted membranes.  Ann N Y Acad Sci  834, 149-152.

11.    Sambongi, Y., Wakabayashi, T. , Yoshimizu, T. , Omote, H. , Oka, T. and Futai, M.  (1997) Caenorhabditis elegans cDNA for Menkes/Wilson disease gene homologue and its function in yeast CCC2 gene deletion mutant.  J. Biochem. 121, 1169-1175.

12.    Takeyasu, K., Omote, H., Nettikadan, S. , Tokumasu, F. , Iwamoto-Kihara, A. and Futai, M. (1996) Molecular imaging of Escherichia coli F_oF₁-ATPases in reconstituted membranes using atomic force microscopy.  FEBS Lett. 392, 110-113.

13.    Futai, M. and Omote, H.  (1996) Conformation transmission in ATP synthase during catalysis: Search for large structural change.  J. Bioenerg. Biomemb. 28, 409-414.

14.    Futai, M. and Omote, H. (1996)  F-type H⁺ATPase: catalytic site and energy coupling.  Handbook of Biological Physics Vol.2, 49 - 74.

15.    Omote, H., Le, N. P., Park, M.-Y., Maeda, M. and Futai, M.  (1995) β subunit Glu-185 of Escherichia coli H⁺-ATPase (ATP synthase) is an essential residue for cooperative catalysis.  J. Biol. Chem.  270, 25656 - 25660.

16.    Jeanteur-De Beukelaer, C., Omote, H., Iwamoto-Kihara, A., Maeda, M. and Futai, M. (1995) β/γ subunit interaction is required for catalysis by H⁺-ATPase (ATP synthase): βsubunit amino acid replacements suppressγ subunit mutation having a long unrelated carboxyl terminal.  J. Biol. Chem.  270, 22850 - 22854.

17.    Futai, M., Omote, H.  and Maeda, M. (1995)  Escherichia coli H⁺-ATPase (ATP synthase): catalytic site and roles of subunit interactions in energy coupling.  Biochem. Soc. Trans. 23, 785 - 789.

18.    Omote, H., Park, M.-Y. , Maeda, M. and Futai, M.  (1994)  The α/β subunit interaction in H⁺-ATPase (ATP synthase): an Escherichia coli α subunit mutation (αArg 296→Cys) restored coupling efficiency to the deleterious by subunit mutant (βSer174®Phe).  J. Biol. Chem. 269, 10265-10269.

19.    Park, M.-Y., Omote, H., Maeda, M. , Futai, M.  (1994) Conserved Glu-181 and Arg-182 residues of Escherichia coli H⁺ -ATPase (ATP synthase) β- subunit are essential for catalysis:Properties of 33 mutants betweenβGlu-161 and β Lys -201 residues.  J. Biochem. 116, 1139-1145.

20.    Futai, M., Park, M.-Y., Iwamoto, A., Omote, H. and Maeda, M.  (1994) Catalysis and energy coupling of H⁺-ATPase (ATP Synthase): Molecular biological approaches. Biochim. Biophys. Acta 1187, 165-170.

21.    Nakamoto, R. K., Shin, K., Iwamoto, A., Omote, H., Maeda, M. and Futai, M.  (1992)  Escherichia coli F_oF₁-ATPase: Residues involved in catalysis and coupling.  Anal. N. Y. Acad. Sci. 671, 335-344.

22.    Futai, M., Iwamoto, A., Omote, H., Maeda, M.  (1992) A glycine-rich sequence in the catalytic site of F-type ATPase.  J. Bioenerg. Biomembr. 24, 463-467.

23.    Omote, H., Maeda, M. and Futai, M.  (1992)  Effects of mutations of conserved Lys-155 and Thr-156 residues in the phosphate binding glycine-rich sequence of the F₁-ATPase βsubunit of Escherichia coli.  J. Biol. Chem. 267, 20571-20576.

24.    Futai, M., Iwamoto, A., Omote, H., Orita, Y., Shin, K., Nakamoto R.K. and Maeda, M.  (1992) Escherichia coli  ATP synthase (F-ATPase) :catalytic site and regulation of H⁺ translocation.  J. Exp. Biol. 172, 443-449.

25.    Iwamoto, A., Omote, H., Hanada, H., Tomioka, N., Itai, A., Maeda, M. and Futai, M. (1991)  Mutations in Ser174 and the glycine-rich sequence (Gly149, Gly150, and Thr156) in the βsubunit of Escherichia coli H⁺-ATPase.  J. Biol. Chem. 266, 16350 - 16355.