S. Nishiyama, Y. Takemoto, K. Yamanouchi, K. Kondo, S. Kawatsu, M. Maruyama, K. Higaki. Dynamic changes in the distribution equilibrium of drugs in nanoemulsions associated with drug absorption facilitate the absorption improvement for poorly water-soluble drugs by SNEDDS. Int. J. Pharm., 674, 125458 (2025)

M. Maruyama, R. Torii, H. Matsui, H. Hayashi, K. Ogawara, K. Higaki. Repeated sequential administration of pegylated emulsion of SU5416 and liposomal paclitaxel enhances anti-tumor effect in 4T1 breast cancer-bearing mice. Eur. J. Pharm. Biopharm., 209, 114663 (2025)

M. Maruyama, T. Ueda, Y. Ienaka, H. Tojo, K. Hyodo, K. Ogawara, K. Higaki. EGF-induced P-gp expression in tumor vasculature contributes to therapeutic resistance to doxorubicin-PEG-liposomes in mice bearing doxorubicin-resistant B16-BL6 tumors. J. Drug Deliv. Sci. Technol., 106, 106690 (2025)

S. Aikawa, H. Tanaka, H. Ueda, M. Maruyama, K. Higaki. Specific intermolecular interaction with sodium glycocholate generates the co-amorphous system showing higher physical stability and aqueous solubility of Y5 receptor antagonist of neuropeptide Y, a brick dust molecule. Eur J Pharm Biopharm., 202, 114395 (2024)

M. Maruyama, H. Matsui, H. Nakamura, R. Torii, Y. Takasugi, K. Ogawara, K. Higaki. Vascular normalization with pegylated emulsion of SU5416 enhances anti-tumor effect of liposomal paclitaxel in 4T1 breast cancer-bearing mice: Analysis of intratumoral vessels and microenvironment. J. Drug Deliv. Sci. Technol., 96, 105647 (2024)

T. Inoue, S. Maehara, M. Maruyama, K. Higaki. Combination of co-amorphization with SNEDDS outperforms Ofev® in the oral absorption of nintedanib. Int J Pharm., 657, 124197 (2024)

H. Mukai, M. Takanashi, K. Ogawara, M. Maruyama, K. Higaki. Possible Regulation of P-Glycoprotein Function by Adrenergic Agonists II: Study with Isolated Rat Jejunal Sheets and Caco-2 Cell monolayers. J Pharm Sci., 113 (5), 1209-1219 (2024).

T. Shehata, Y. Kono, K. Higaki, T. Kimura and K. Ogawara:　In vivo distribution characteristics and anti-tumor effect of doxorubicin encapsulated in PEG-modified niosomes in solid tumor-bearing mice. J. Drug Deliv. Sci. and Technol., 80, 104122 (2023).

Y. Takamura, S. Kikuzawa, M. Fujihara, Y. Sunatsuki, H. Ota, K. Higaki and H. Kakuta: Characterization and interconversion of two crystal forms of Net-3IB, a retinoid X receptor agonist. Chem. Pharm. Bull., 71 (4), 282-288 (2023).

M. Gomi, N. Mizutani, R. Senoo, N. Matsubara, A. Watanabe, M. Maruyama, G. Kimura, K. Higaki. Development of Sustained Release System Based on High Water-Absorbable Gel Formation Using Croscarmellose Sodium, Alkaline Excipients and HPMC (ACSH SR System); Novel Application of Croscarmellose Sodium as a Gel Former. Pharm Res., 40, 3073-3086 (2023)

M. Maruyama, M. Yoshikata, M. Sakaguchi, S. Wakushima, K. Higaki. Establishment of a novel in vitro co-culture system of enteric neurons and Caco-2 cells for evaluating the effect of enteric nervous system on transepithelial transport of drugs. Int. J. Pharm., 633, 122617 (2023)

K. Yamanouchi, T. Ishimaru, T. Kakuno, Y. Takemoto, S. Kawatsu, K. Kondo, M. Maruyama, K. Higaki. Improvement and characterization of oral absorption behavior of clofazimine by SNEDDS: Quantitative evaluation of extensive lymphatic transport. Eur. J. Pharm. Biopharm., 187, 141-155 (2023).

S. Aikawa, H. Tanaka, H. Ueda, M. Maruyama, K. Higaki. Formation of a Stable Co-Amorphous System for a Brick Dust Molecule by Utilizing Sodium Taurocholate with High Glass Transition Temperature. Pharmaceutics., 15, 84 (2023).

S. Nakashima, T. Iwamoto, M. Takanashi, K. Ogawara, M. Maruyama, K. Higaki: Effect of Excessive Serotonin on Pharmacokinetics of Cephalexin after Oral Administration: Studies with Serotonin-Excessive Model Rats: Pharm. Res., 39, 2163-2178 (2022).

M. Onishi, K. Tagawa, M. Jiko, K. Koike, M. Maruyama, H. Hashizume, K. Imagaki, K. Higaki: Efficient Evaluation of In Vivo Performance in Human for Generic Formulation by Novel Dissolution-Absorption Prediction (DAP) Workflow: Pharm. Res., 39, 2203-2216 (2022).

M. Maruyama, Y. Nishida, H. Tanaka, T. Minami, K.I. Ogawara, M. Miyake, Y. Takamura, H. Kakuta, K. Higaki: Analysis of absorption-enhancing mechanisms for combinatorial use of spermine with sodium taurocholate in Caco-2 cells. Eur. J. Pharm. Biopharm., 180, 332-343 (2022).

Y. Kono, A. Fushimi, Y. Yoshizawa, K. Higaki and K. Ogawara: Effects of particle size and release property of paclitaxel-loaded nanoparticles on their peritoneal retention and therapeutic efficacy against mouse malignant ascites: Int. J. Pharm., 623, 121904 (2022).

Y. Sumimoto, S. Okawa, T. Inoue, K. Masuda, M. Maruyama, K. Higaki. Extensive improvement of oral bioavailability of mebendazole, a brick dust, by polymer-containing SNEDDS preparation: Disruption of high crystallinity by utilizing its counter ion. Eur. J. Pharm. Biopharm.,172, 213-227 (2022)

M. Maruyama, H. Tojo, K. Toi, Y. Ienaka, K. Hyodo, H. Kikuchi, K. Ogawara, and K. Higaki: Effect of Doxorubicin Release Rate From Polyethylene Glycol-Modified Liposome on Anti-tumor Activity in B16-BL6 Tumor-Bearing Mice. J. Pharm. Sci., 111, 293-297, (2022).

T. Minamisakamoto, S. Nishiguchi, K. Hashimoto, K. Ogawara, M. Maruyama, and K. Higaki: Sequential administration of PEG-Span 80 niosome enhances anti-tumor effect of doxorubicin-containing PEG liposome. Eur. J. Pharm. Biopharm., 169, 20-28 (2021).

H. Mukai, M. Takanashi, K. Ogawara, M. Maruyama, and K. Higaki: Possible Regulation of P-glycoprotein Function by Adrenergic Agonists in a Vascular-luminal Perfused Preparation of Small Intestine. J. Pharm. Sci., 110 (12), 3889-3895 (2021).

S. Okawa, Y. Sumimoto, K. Masuda, K. Ogawara, M. Maruyama and K. Higaki: Improvement of lipid solubility and oral bioavailability of a poorly water- and poorly lipid-soluble drug, rebamipide, by utilizing its counter ion and SNEDDS preparation. Eur. J. Pharm. Sci., 159, 105721 (2021).

M. Miyake, T. Minami, M. Maruyama, T. Mukai and K. Higaki: Spermine with sodium taurocholate enhances pulmonary absorption of macromolecules in rats. J. Pharm. Sci., 110 (10), 3464-3470 (2021).

M. Maruyama, Y. Nakano, T. Nishimura, R. Iwata, S. Matsuda, M. Hayashi, Y. Nakai, M. Nonaka and T. Sugimoto: PC3-secreted microprotein is expressed in glioblastoma stem-like cells and human glioma tissues. Biol. Pharm. Bull., 44 (7), 910-919 (2021).

K. Kohara, A. Inoue, Y. Nakano, H. Hirai, T. Kobayashi, M. Maruyama, R. Baba and C. Kawashima: BATTLE: Genetically Engineered Strategies for Split-Tunable Allocation of Multiple Transgenes in the Nervous System. iScience, 23 (6), 101248 (2020).

R. Iwata, J. Hyoung Lee, M. Hayashi, U. Dianzani, K. Ofune, M. Maruyama, S. Oe, T. Ito, T. Hashiba, K. Yoshimura, M. Nonaka, Y. Nakano, Norian L., I. Nakano and A. Asai: ICOSLG-mediated regulatory T-cell expansion and IL-10 production promote progression of glioblastoma. Neuro. Oncol., 22 (3), 333-344 (2020).

K. Okada, A. Sato, A. Hiramoto, R. Isogawa, Y. Kurosaki, K. Higaki, S-I. Miyoshi, K-S. Chang and H-S. Kim: Pharmacokinetic analysis of new synthetic antimalarial N-251. Trop. Med. Health, 47, 40 (2019).

D. Inoue, A. Tanaka, S. Kimura, A. Kiriyama, H. Katsumi, A. Yamamoto, K. Ogawara, T. Kimura, K. Higaki, R. Yutani, T. Sakane, T. Furubayashi: The relationship between in vivo nasal drug clearance and in vitro nasal mucociliary clearance: Application to the prediction of nasal drug absorption: Eur. J. Pharm. Sci., 117, 21-26 (2018).

D. Inoue, S. Kimura, A. Kiriyama, H. Katsumi, A. Yamamoto, K. Ogawara, K. Higaki, A. Tanaka, R. Yutani, T. Sakane, T. Furubayashi: Quantitative estimation of the effect of nasal mucociliary function on in vivo absorption of norfloxacin after intranasal administration to rats: Mol. Pharm., 15 (10), 4462-4469 (2018).

The involvement of cancer stem cells in brain metastatic process. R. Iwata, M. Maruyama, K. Ofune, Y. Nakano, S. Oe, M. Hayashi, K. Yoshimura, M. Nonaka and A Asai: Cytometry Res., 28 (1), 13-18 (2018).

R. Iwata, M. Maruyama, T. Ito, Y. Nakano, Y. Kanemura, T. Koike, S. Oe, K. Yoshimura, M. Nonaka, S. Nomura, T. Sugimoto, H. Yamada and A. Asai: Establishment of a tumor sphere cell line from a metastatic brain neuroendocrine tumor. Med. Mol. Morphol., 50 (4), 211-219 (2017).

S. Kozai, T. Wada, K. Ogawara, T. Kida, H. Tokushige and K. Higaki: Evaluation of pharmacokinetic-pharmacodynamic indices of topical ophthalmic gatifloxacin against Staphylococcus aureus utilizing an in-vitro aqueous humour pharmaco- kinetic (AHPK) model. Int. J. Antimicrobial Agents, 49, 113-115 (2017).

K. Ogawara, Y. Fukuoka, Y. Yoshizawa, T. Kimura and K. Higaki: Development of safe and potent oil-in-water emulsion of paclitaxel to treat peritoneal dissemination. J. Pharm. Sci., 106, 1143-1148 (2017).

D. Tsunashima, K. Yamashita, K. Ogawara, K. Sako, T. Hakomori and K. Higaki: Development of extended release solid dispersion granules of tacrolimus: Evaluation of release mechanism and human oral bioavailability: J. Pharm. Pharmacol., 69, 1697-1706 (2017).

K. Ogawara and K. Higaki: Nanoparticle-based photodynamic theraphy: Current status and future application to improve outcomes of cancer treatment: Chem. Pharm. Bull., 65 (7) 637-641 (2017).
　
K. Ogawara, T. Shiraishi, T. Araki, T. Watanabe, T. Ono and K. Higaki: Efficient anti-tumor effect of photodynamic treatment with polymeric nanoparticles composed of polyethylene glycol and polylactic acid block copolymer encapsulating hydrophobic porphyrin derivative. Eur. J. Pharm. Sci., 82 154-160 (2016).

D. Tsunashima, K. Yamashita, K. Ogawara, K. Sako, and K. Higaki: Preparation of extended release solid dispersion formulations of tacrolimus using ethylcellulose and hydroxypropylmethylcellulose by solvent evaporation method. J. Pharm. Pharmacol., 68 (3) 316-323 (2016).
　
T. Shehata, T. Kimura, K. Higaki and K. Ogawara: In-vivo disposition characteristics of PEG niosome and its interaction with serum proteins. Int. J. Pharm., 512, 322-328 (2016).

H. Kamiguchi, M. Murabayashi, I. Mori, A. Horinouchi and K. Higaki: Biomarker discovery for drug-induced phospholipidosis: phenylacetylglycine to hippuric acid ratio in urine and plasma as potential markers. Biomarkers, 22, 178-188 (2016).

T. Araki, K. Ogawara, H. Suzuki, R. Kawai, T. Watanabe, T. Ono and K. Higaki: Augmented EPR effect by photo-triggered tumor vascular treatment improved therapeutic efficacy of liposomal paclitaxel in mice bearing tumors with low permeable vasculature. J. Controlled Rel., 200 (1) 106-114 (2015).
　
C. Imada, T. Takahashi, M. Kuramoto, K. Masuda, K. Ogawara, A. Sato, Y. Wataya, H-S. Kim and K. Higaki: Improvement of oral bioavailability of N-251, a novel antimalarial drug, by increasing lymphatic transport with long-chain fatty acid-based self-nanoemulsifying drug delivery system. Pharm. Res., 32 (8) 2595-2608 (2015).
　
M. Sugihara, S. Takeuchi, M Sugita, K. Higaki, M. Kataoka and S. Yamashita: Analysis of intra- and inter-subject variability in oral drug absorption in human bioequivalence studies of 131 generic products. Mol. Pharm., 12 (12) 4405-4413 (2015).

A. Elgaml, K. Higaki and S. Miyoshi: Effects of temperature, growth phase and luxO-disruption on regulation systems of toxin production in Vibrio vulnificus strain L-180, a human clinical isolate. World J. Microbiol. Biotechnol., 30 (2) 681-691 (2014).

M. Naruse, K. Ogawara, T. Kimura, R. Konishi and K. Higaki: Development of transdermal therapeutic formulation of CNS5161, a novel NMDA receptor antagonist, by utilizing pressure-sensitive adhesives II: Improved transdermal absorption and evaluation of efficacy and safety. Eur. J. Pharm. Sci., 52 86-94 (2014).

H. Tani, S. Hikami, S. Iiduna, M. Yoshimatsu, T. Asama, H. Ota, Y. Kimura, T. Tatefuji, K. Hashimoto and K. Higaki: Pharmacokinetics and Safety of Resveratrol Derivatives in Humans after Oral Administration of Melinjo (Gnetum gnemon L.) Seed Extract Powder. J. Agric. Food Chem., 62 (9) 1999-2007 (2014).

D. Tsunashima, A. Kawamura, M. Murakami, T. Sawamoto, N. Undre, M. Brown, A. Groenewoud, J. Keirns, J. Holman, A. Connor, H. Wylde, I. Wilding, K. Ogawara, K. Sako, K. Higaki and R. First: Assessment of Tacrolimus Absorption from the Human Intestinal Tract: Open Label, Randomized, Four-way Crossover Study. Clin. Ther., 36 (5) 748-759 (2014).

三宅 正晃、向井 正志、檜垣 和孝: 小腸における難吸収性薬物の吸収改善に関する検討(Absorption Improvement of Poorly Absorbable Drug from Small Intestine): J. Pharm. Sci. Technol. Japan (薬剤学), 74 (5) 361-365 (2014).

K. Ogawara, S. Abe, K. Un, Y. Yoshizawa, T. Kimura and K. Higaki: Determinants for in vivo anti-tumor effect of angiogenesis inhibitor SU5416 formulated in PEGylated emulsion. J. Pharm. Sci., 103 (8) 2464-2469 (2014).

Y. Yoshizawa, K. Ogawara, T. Kimura and K. Higaki: A novel approach to overcome multidrug resistance: Utilization of P-gp mediated efflux of paclitaxel to attack neighboring vascular endothelial cells in tumors. Eur. J. Pharm. Sci., 62 274-280 (2014).

M. Miyake, H. Toguchi, T. Nishibayashi, K. Higaki, A. Sugita, K. Koganei, N. Kamada, N-T. Kitazume, T. Hisamatsu, T. Sato, S. Okamoto, T. Kanai and T. Hibi: Establishment of novel prediction system of intestinal absorption in humans using human intestinal tissues. J. Pharm. Sci., 102 (8) 2564-2571 (2013).

D. Inoue, T. Furubayashi, K. Ogawara, T. Kimura, K. Higaki, T. Shingaki, S. Kimura, A. Tanaka, H. Katsumi, T. Sakane, A. Yamamoto and Y. Higashi: In vitro evaluation of the ciliary beat frequency of the rat nasal epithelium using a high-speed digital imaging system. Biol. Pharm. Bull., 36 (6) 966-973 (2013).

S. Takemura, H. Kondo, S. Watanabe, K. Sako, K. Ogawara and K. Higaki: Aminoalkylmethacrylate copolymer E improves oral bioavailability of YM466 by suppressing drug – bile Interaction. J. Pharm. Sci., 102 (9) 3128-3135 (2013).

A. Elgaml, K. Higaki and S. Miyoshi: Regulation system of serine protease production in Vibrio vulnificus strain NCIMB 2137, a metalloprotease-gene negative strain isolated from a diseased eel. Aquaculture, 416-417 315-321 (2013).

K. Ogawara, Y, Yoshizawa, K. Un T. Araki, T. Kimura and K. Higaki: Nanoparticle-based passive drug targeting to tumors: Considerations to be given and implications for further optimization: Biol. Pharm. Bull., 36 (5) 398 – 402 (2013).

M. Naruse, K. Ogawara, T. Kimura, R. Konishi and K. Higaki: Development of transdermal therapeutic formulation of CNS5161, a novel NMDA receptor antagonist, by utilizing pressure-sensitive adhesives I. Biol. Pharm. Bull., 35 (3) 321-328 (2012).

S. Takemura, H. Kondo, K. Suzumura, K. Ogawara, S. Watanabe, K. Sako and K. Higaki: Evaluation of factors affecting gastrointestinal absorption of a novel anticoagulant FX-93 for development of oral formulation. J. Pharm. Sci., 101 (6) 2134-2142 (2012).

D. Inoue, T. Furubayashi, K. Ogawara, T. Kimura, K. Higaki, H. Katsumi, T. Sakane, A. Yamamoto and Y. Higashi: In vitro evaluation of nasal mucociliary clearance using excised rat nasal septum. Biol. Pharm. Bull., 35 (6) 889-894 (2012).

T. Araki, Y. Kono, K. Ogawara, T. Watanabe, T. Ono, T. Kimura and K. Higaki: Formulation and evaluation of paclitaxel-loaded polymeric nanoparticles composed of polyethylene glycol and polylactic acid block copolymer. Biol. Pharm. Bull., 35 (8) 1306-1313 (2012).

Y. Miyaji, Y. Kasuya, Y. Furuta, A. Kurihara, M. Takahashi, K. Ogawara, T. Izumi, O. Okazaki and K. Higaki: Novel comb-shaped PEG modification enhances the osteoclastic inhibitory effect and bone delivery of osteoprotegerin after intravenous administration in ovariectomized rats. Pharm. Res., 29 (11) 3143-3155 (2012).

Y. Yoshizawa, K. Ogawara, A. Fushimi, S. Abe, K. Ishikawa,T. Araki, G. Molema, T. Kimura and K. Higaki: Deeper penetration into tumor tissues and enhanced in-vivo anti-tumor activity of liposomal paclitaxel by pre-treatment with angiogenesis inhibitor SU5416. Mol. Pharm., 9 (12) 3486-3494 (2012).

T. Kanda, T. Harada, Y. Tominaga, K. Suzumori, T. Ono, S. Iwabuchi, K. Ito, K. Ogawara, K. Higaki and Y. Yoshizawa: The Design and evaluation of emulsion generation device using ultrasonic vibration and microchannel. Jpn. J. Appl. Phys., 50 07HE24 -1 – 07HE24‐6 (2011).

Y. Yoshizawa, Y. Kono, K. Ogawara, T. Kimura and K. Higaki: PEG liposomalization of paclitaxel improved its in-vivo disposition and anti-tumor efficacy. Int. J. Pharm., 412 (1-2) 132-141 (2011).

A. Sato, A. Hiramoto, M. Morita, M. Matsumoto, Y. Komichi, Y. Nakase, N. Tanigawa, O. Hiraoka, K. Hiramoto, H. Hayatsu, K. Higaki, A. Kawai, A. Masuyama, M. Nojima, Y. Wataya and H.-S. Kim: Antimalarial activity of endoperoxide compound 6-(1,2,6,7-tetraoxaspiro[7.11]nonadec-4-yl)hexan-1-ol. Parasitol. Int., 60 (3) 270-273 (2011).

N. Kamada, K. Yamada, M. Odomi, T. Mukai, T. Nishibayashi, K. Ogawara, T. Kimura and K. Higaki: Sex differences in pharmacokinetics of cilostazol in rats. Xenobiotica, 41 (10) 903-913 (2011).

A. Sato, S. Kawai, A. Hiramoto, M. Morita, N. Tanigawa, Y. Nakase, Y. Komichi, M. Matsumoto, O. Hiraoka, K. Hiramoto, H. Tokuhara, A. Masuyama, M. Nojima, K. Higaki, H. Hayatsu, Y. Wataya and H.-S. Kim: Antimalarial activity of 6-(1,2,6,7-tetraoxaspiro[7.11]nonadec-4-yl)hexan-1-ol (N-251) and its carboxylic acid derivatives. Parasitol. Int., 60 (4) 488-492 (2011).

T. Harada, T. Kanda, O. Suzumori, T. Ono, S. Iwabuchi, K. Ito, K. Ogawara and K. Higaki: Emusion generating microchannel device oscillated by 2.25 MHz ultrasonic vibrator. Jpn. J. Appl. Phys., 49 07HE13-1 - 07HE13-6 (2010).

檜垣 和孝、木村聰城郎: 自己乳化型マイクロエマルション製剤: J. Pharm. Sci. Technol. Japan. (薬剤学) 70 (1) 32-38 (2010).

T. Hironaka, S. Itokawa, K. Ogawara, K. Higaki and T. Kimura: Quantitative evaluation of PEPT1 contribution to oral absorption of cephalexin in rats. Pharm. Res., 26 (1) 40-50 (2009).

K. Ogawara, K. Un, K. Tanaka, K. Higaki and T. Kimura: In vivo anti-tumor effect of PEG liposomal doxorubicin (DOX) in DOX-resistant tumor-bearing mice: Involvement of cytotoxic effect on vascular endothelial cells. J. Controlled Rel., 133 (1) 4-10 (2009).

F. Mukaizawa, K. Taniguchi, M. Miyake, K. Ogawara, M. Odomi, K. Higaki and T. Kimura: Novel oral absorption system containing polyamines and bile salts enhances drug transport via both transcellular and paracellular pathways across Caco-2 cell monolayers. Int. J. Pharm., 367 (1-2) 103-108 (2009).

T. Kimoto, M. Takanashi, H. Mukai, K. Ogawara, T. Kimura and K. Higaki: Effect of adrenergic stimulation on drug absorption via passive diffusion in Caco-2 cells. Int. J. Pharm., 368 (1-2) 31-36 (2009).

C. Takayama, F. Mukaizawa, T. Fujita, K. Ogawara, K. Higaki and T. Kimura: Amino acids suppress apoptosis induced by sodium laurate, an absorption enhancer. J. Pharm. Sci., 98 (12) 4629-4638 (2009).

檜垣 和孝: オーラルメディケーションにおける胃排出の重要性: Pharm Tech Japan 25 (13) 48-52 (2009).

檜垣 和孝: 自己乳化型マイクロエマルション製剤による吸収改善: Farumashia 45 (12) 1213-1216 (2009).

J. Yokoe, S. Sakuragi, K. Yamamoto, T. Teragaki, K. Ogawara, K. Higaki, N. Katayama, T. Kai, M. Sato and T. Kimura: Albumin-conjugated PEG liposome enhances tumor distribution of liposomal doxorubicin in rats. Int. J. Pharm., 353 (1-2) 28-34 (2008).

M. Ishii, Y. Kawakami, H. Date, K. Higaki, T. Sendo and T. Kimura: Correlation of cyclosporine blood concentration-dose ratio and biochemical parameters in living-donor lung transplant patients. Jpn. J. Pharm. Healthcare Sci., 34 (4) 381-385 (2008).

H. Wakamatsu, S. Nagamachi, R. Nishii, K. Higaki, K. Kawai, K. Kamimura, S. Fujita, S. Futami and S. Tamura: Effect of percutaneous endoscopic gastrostomy on gastrointestinal motility: evaluation by gastric-emptying scintigraphy. Nucl. Med. Commun., 29 (6) 562-567 (2008).

K. Higaki, S. Y. Choe, R. Löbenberg, L. S. Welage and G. L. Amidon: Mechanistic understanding of time-dependent oral absorption based on gastric motor activity in humans. Eur. J. Pharm. Biopharm., 70 (1) 313-325 (2008).

K. Ogawara, K. Un, K. Minato, K. Tanaka, K. Higaki and T. Kimura: Determinants for in-vivo anti-tumor effects of PEG liposomal doxorubicin: Importance of vascular permeability within tumors. Int. J. Pharm., 359 (1-2) 234-240 (2008).

T. Shehata, K. Ogawara, K. Higaki and T. Kimura: Prolongation of residence time of liposome by surface-modification with mixture of hydrophilic polymers. Int. J. Pharm., 359 (1-2) 272-279 (2008).

J. Jinno, N. Kamada, M. Miyake, K. Yamada, T. Mukai, M. Odomi, H. Toguchi, G. G. Liversidge, K. Higaki and T. Kimura: In vitro-in vivo correlation for wet-milled tablet of poorly water-soluble cilostazol. J. Controlled Rel., 130 (1) 29-37 (2008).

Y. Metsugi, Y. Miyaji, K. Ogawara, K. Higaki and T. Kimura: Appearance of double peaks in plasma concentration – time profile after oral administration depends on gastric emptying profile and weight function. Pharm. Res., 25 (4) 886-895 (2008).

Y. Fujioka, Y. Metsugi, K. Ogawara, K. Higaki and T. Kimura: Evaluation of in-vivo dissolution behavior and GI transit of griseofulvin, a BCS class II drug. Int. J. Pharm., 352 (1-2) 36-43 (2008).

K. Furumoto, J. Yokoe, K. Ogawara, S. Amano, M. Takaguchi, K. Higaki, T. Kai and T. Kimura: Effect of coupling of albumin onto surface of PEG liposome on its in-vivo disposition. Int. J. Pharm., 329 (1-2) 110-116 (2007).

S. Nagayama, K. Ogawara, K. Minato, Y. Fukuoka, Y. Takakura, M. Hashida, K. Higaki and T. Kimura: Fetuin mediates hepatic uptake of negatively charged nanoparticles via scavenger receptor. Int. J. Pharm., 329 (1-2) 192-198 (2007).

N. Nagahara, Y. Akiyama, K. Higaki and T. Kimura: Animal models for predicting potency of oral sustained-release adhesive microspheres in humans. Int. J. Pharm., 331 (1) 46-53 (2007).

Y. Fujioka, K. Kadono, Y. Fujie, Y. Metsugi, K. Ogawara, K. Higaki and T. Kimura: Prediction of oral absorption of griseofulvin, a BCS class II drug, based on GITA model: Utilization of a more suitable medium for in-vitro dissolution study. J. Controlled Rel., 119 (2) 222-228 (2007).

S. Nagayama, K. Ogawara, Y. Fukuoka, K. Higaki and T. Kimura: Time-dependent changes in opsonins amount associated on nanoparticles alter their hepatic characteristics. Int. J. Pharm., 342 (1-2) 215-221 (2007).

檜垣 和孝: アミノ酸とラウリン酸を利用した安全性の高い吸収改善製剤の開発: アミノ酸によるcytoprotectionとその機構 (Safe improvement of drug absorption by combinatorial use of sodium laurate with amino acids: Cytoprotection by amino acids and its mechanisms). 薬学雑誌 (Journal of the Pharmaceutical Society of Japan) 127 (4) 589-599 (2007).

M. Miyake, T. Minami, M. Hirota, H. Toguchi, M. Odomi, K. Ogawara, K. Higaki and T. Kimura: Novel oral formulation safely improving intestinal absorption of poorly absorbable drugs: Utilization of polyamines and bile acids. J. Controlled Rel., 111 (1-2) 27-34 (2006).

J. Jinno, N. Kamada, M. Miyake, K. Yamada, T. Mukai, M. Odomi, H. Toguchi, G. G. Liversidge, K. Higaki and T. Kimura: Effect of particle size reduction on dissolution and oral absorption of a poorly water-soluble drug, cilostazol, in beagle dogs. J. Controlled Rel., 111 (1-2) 56-64 (2006).

M. Miyake, T. Minami, Y. Oka, N. Kamada, H. Yamazaki, Y. Kato, T. Mukai, H. Toguchi, M. Odomi, K. Ogawara, K. Higaki and T. Kimura: Optimization of suppository preparation containing sodium laurate and taurine that can safely improve rectal absorption of rebamipide. Biol. Pharm. Bull., 29 (2) 330-335 (2006).

N. Tanaka, K. Imai, K. Okimoto, S. Ueda, Y. Tokunaga, R. Ibuki, K. Higaki and T. Kimura: Development of novel sustained-release system, disintegration-controlled matrix tablet (DCMT) with solid dispersion granules of nilvadipine (II): in vivo evaluation. J. Controlled Rel., 112 (1) 51-56 (2006).

T. Okuda, K. Kadotsuji, C. Takayama, K. Hanada, F. Mukaizawa, K. Ogawara, K. Higaki and T. Kimura: Involvement of intracellular Ca2+ dynamics in cytoprotective action by amino acids and cytotoxicity by sodium laurate, an absorption enhancer. J. Pharm. Sci., 95 (10) 2256-2265 (2006).

M. Miyake, T. Minami, H. Toguchi, M. Odomi, K. Ogawara, K. Higaki and T. Kimura: Importance of bile acids for novel oral absorption system containing polyamines to improve intestinal absorption. J. Controlled Rel., 115 (2) 130-133 (2006).

N. Tanaka, K. Imai, K. Okimoto, S. Ueda, Y. Tokunaga, A. Ohike, R. Ibuki, K. Higaki and T. Kimura: Development of novel sustained-release system, disintegration-controlled matrix tablet (DCMT) with solid dispersion granules of nilvadipine. J. Controlled Rel., 108 (2-3) 386-395 (2005).

K. Higaki, K. Nakayama, T. Suyama, C. Amnuaikit, K. Ogawara and T. Kimura: Enhancement of topical delivery of drugs via direct penetration by reducing blood flow rate in skin. Int. J. Pharm., 288 (2), 227-233 (2005).

H. Hiraoka, N. Kimura, Y. Furukawa, K. Ogawara, T. Kimura and K. Higaki: Upregulation of P-glycoprotein expression in small intestine under chronic serotonin-depleted conditions in rats. J. Pharmacol. Exp. Ther., 312 (1), 248-255 (2005).

C. Amnuaikit, I. Ikeuchi, K. Ogawara, K. Higaki and T. Kimura: Skin permeation of propranolol from polymeric film containing terpene enhancers for transdermal use. Int. J. Pharm., 289 (1-2), 167-178 (2005).

T. Takatori, K. Yamamoto, T. Yamaguchi, K. Higaki and T. Kimura: Design of controlled-release morphine suppositories containing polyglycerol ester of fatty acid. Biol. Pharm. Bull., 28 (8) 1480-1484 (2005).

K. Furumoto, S. Nagayama, K. Ogawara, Y. Takakura, M. Hashida, K. Higaki and T. Kimura: Hepatic uptake of negatively charged particles in rats: Possible involvement of serum proteins in recognition by scavenger receptor. J. Controlled Rel., 97 (1), 133-141 (2004).

C. Nishida, K. Ogawara, T. Kimura and K. Higaki: Pharmacokinetic analysis of factors determining elimination pathways for sulfate and glucuronide metabolites of xenobiotics III: Mechanisms for sinusoidal efflux of 4-methylumbelliferone sulfate. Xenobiotica, 34 (5) 439-448 (2004).

K. Higaki, M. Sone, K. Ogawara and T. Kimura: Regulation of drug absorption from small intestine by enteric nervous system I: a poorly absorbable drug via passive diffusion. Drug Metab. Pharmacokinet., 19 (3) 198-205 (2004).

T. Takatori, N. Shimono, K. Higaki and T. Kimura: Evaluation of sustained release suppositories prepared with fatty base including solid fats with high melting points. Int. J. Pharm., 278 (2), 275-282 (2004).

M. Miyake, N. Kamada, Y. Oka, T. Mukai, T. Minami, H. Toguchi, M. Odomi, K. Ogawara, K. Higaki and T. Kimura: Development of suppository formulation safely improving rectal absorption of rebamipide, a poorly absorbable drug, by utilizing sodium laurate and Taurine. J. Controlled Rel., 99 (1), 63-71 (2004).

K. Ogawara, K. Furumoto, S. Nagayama, K. Minato, K. Higaki, T. Kai and T. Kimura: Pre-coating with serum albumin reduces receptor-mediated hepatic disposition of polystyrene nanosphere: Implications for rational design of nanoparticles. J. Controlled Rel., 100 (3), 451-455 (2004).

J. Yokoe, N, Iwasaki, S. Haruta, K. Kadono, K. Ogawara, K. Higaki and T. Kimura: Analysis and prediction of absorption behavior of colon-targeted prodrug in rats by Gastrointestinal-Transit-Absorption (GITA) model. J. Controlled Rel., 86 (2-3) 305-313 (2003).

K. Nezasa, K. Higaki, M. Takeuchi, M. Nakano and M. Koike: Uptake of rosuvastatin by isolated rat hepatocytes: Comparison with pravastatin. Xenobiotica, 33 (4) 379-388 (2003).

M. Miyake, Y. Oka, T. Minami, H. Toguchi, M. Odomi, K. Ogawara, K. Higaki and T. Kimura: Combinatorial use of sodium laurate with taurine or L-glutamine enhances colonic absorption of rebamipide, poorly absorbable anti-ulcer drug, without any serious histopathological mucosal damage. J. Pharm. Sci., 92 (4) 911-921 (2003).

S. Ohtani, K. Shirasu, K. Ogawara, K. Higaki and T. Kimura: Evaluation of inhibitory activity of casein on proteases in rat intestine. Pharm. Res., 20 (4) 611-617 (2003).

Y-Y Chiu, K. Higaki, B. L. Neudeck, J. L. Barnett, L. S. Welage and G. L. Amidon: Human jejunal permeability of cyclosporin A: Influence of surfactants on P-glycoprotein efflux in Caco-2 cells. Pharm. Res., 20 (5) 749-756 (2003).

S. Ohtani, K Ogawara, K. Higaki and T. Kimura: Casein enhances stability of peptides in intestinal lumen: Role of digested products of casein. Pharm. Res., 20 (11) 1746-1751 (2003).

K. Yamashita, T. Nakate, K. Okimoto, A. Ohike, Y. Tokunaga, R. Ibuki, K. Higaki and T. Kimura: Establishment of new preparation method for solid dispersion formulation of tacrolimus. Int. J. Pharm., 267 (1-2), 79-91 (2003).

K. Higaki, M. Ishii, H. Esumi, M. Kanayama, K. Ogawara and T. Kimura: Pharmacokinetic analysis of factors determining elimination pathways for sulfate and glucuronide Metabolites of xenobiotics II: Studies with isolated perfused rat liver. Xenobiotica, 33 (11) 1097-1108 (2003).

K. Higaki, C. Amnuaikit and T. Kimura: Strategies for overcoming stratum corneum: Chemical and Physical Approaches. Am. J. Drug Deliv., 1 (3) 187-214 (2003).

T. Kimura, H. Yamano, A. Tanaka, T. Matsumura, M. Ueda, K. Ogawara and K. Higaki: Transport of D-glucose across cultured stratified cell layer of human oral mucosal cells. J. Pharm. Pharmacol., 54 (2) 213-219 (2002).

Y. Endo, K. Hanada, M. Miyake, K. Ogawara, K. Higaki and T. Kimura: Mechanisms of cytoprotective effect of amino acids on local toxicity caused by sodium laurate, a drug absorption enhancer, in intestinal epithelium. J. Pharm. Sci., 91 (3) 730-743 (2002).

S. Haruta, K. Kawai, R. Nishii, S. Jinnouchi, K. Ogawara, K. Higaki, S. Tamura, K. Arimori and T. Kimura: Prediction of plasma concentration - time curve of orally administered theophylline based on a scintigraphic monitoring of gastrointestinal transit in human volunteers. Int. J. Pharm., 233 (1-2) 179-190 (2002).

M. Ishii, M. Kanayama, H. Esumi, K. Ogawara, T. Kimura and K. Higaki: Pharmacokinetic analysis of factors determining elimination pathways for sulfate and glucuronide metabolites of drugs I: Studies by in vivo constant infusion. Xenobiotica, 32 (5), 441-450 (2002).

K. Higaki, M. Asai, T. Suyama, K. Nakayama, K. Ogawara and T. Kimura: Estimation of intradermal disposition kinetics of drugs: II. Factors determining penetration of drugs from viable skin to muscular layer. Int. J. Pharm., 239 (1-2), 129-141 (2002).

J. Kubo, Y. Ohigashi, M. Hamabe, K. Ogawara, K. Higaki, H. Saito and T. Kimura: Pharmacokinetic evaluation of high pulmonary disposition of clarithromycin after systemic administration. Xenobiotica, 32 (10), 879-893 (2002).

K. Furumoto, K. Ogawara, S. Nagayama, Y. Takakura, M. Hashida, K. Higaki and T. Kimura: Important role of serum proteins associated on the surface of particles in their hepatic disposition. J. Controlled Rel., 83 (1), 89-96 (2002).

K. Kadono, J. Yokoe, K. Ogawara, K. Higaki and T. Kimura: Analysis and prediction of absorption behavior for theophylline orally administered as powders based on gastrointestinal-transit-absorption (GITA) model. Drug Metab. Pharmacokinet., 17 (4), 307-315 (2002).

K. Nezasa, K. Higaki, T. Matsumura, K. Inazawa, H. Hasegawa, M. Nakano and M. Koike: Liver-specific distribution of rosuvastatin in rats: Comparison with pravastatin and simvastatin. Drug Metab. Dispos., 30 (11) 1158-1163 (2002).

T. Kimura and K. Higaki: Gastrointestinal transit and drug absorption. Biol. Pharm. Bull., 25 (2) 149-164 (2002).

S. Haruta, K. Kawai, S. Jinnouchi, K. Ogawara, K. Higaki, S. Tamura, K. Arimori and T. Kimura: Evaluation of absorption kinetics of orally administered theophylline in rats based on gastrointestinal transit monitoring by gamma scintigraphy. J. Pharm. Sci., 90 (4) 464-473 (2001).

K. Higaki, S. Yamashita and G. L. Amidon: Time-dependent oral absorption models. J. Pharmacokin. Pharmacodyn., 28 (2) 109-128 (2001).

X-Y. Chu, G. P. S-Castaňo, K. Higaki, O. D-Man, C-P. Hsu and G. L. Amidon: Correlation between epithelial cell permeability of cephalexin and expression of intestinal oligopeptide transporter. J. Pharmacol. Exp. Ther., 299 (2) 575-582 (2001).

K. Furumoto, K. Ogawara, M. Yoshida, Y. Takakura, M. Hashida, K. Higaki and T. Kimura: Biliary excretion of polystyrene microspheres depends on the type of receptor-mediated uptake in rat liver. Biochim. Biophys. Acta., 1526 (2) 221-226 (2001).

T. Yata, Y. Endo, M. Sone, K. Ogawara, K. Higaki and T. Kimura: Amino acids protect epithelial cells from local toxicity by absorption enhancer, sodium laurate. J. Pharm. Sci., 90 (8) 1456-1465 (2001).

K. Higaki, T. Yata, M. Sone, K. Ogawara and T. Kimura: Estimation of absorption enhancement by medium-chain fatty acids in rat large intestine. Res. Commun. Mol. Pathol. Pharmacol., 109 (3-4) 231-240 (2001).

K. Ogawara, K. Furumoto, Y. Takakura, M. Hashida, K. Higaki and T. Kimura: Surface hydrophobicity is not necessarily the most important determinant in their in vivo disposition after intravenous administration in rats. J. Controlled Release, 77 (3) 191-198 (2001)

大河原賢一、檜垣 和孝、木村聰城郎: 微粒子キャリアを用いたデリバリーと動態解析. Drug Delivery System 16 (5) 395-400 (2001).

T. Kimura, N. Iwasaki, J. Yokoe, S. Haruta, Y. Yokoo, K. Ogawara and K. Higaki: Analysis and prediction of absorption profile including hepatic first-pass metabolism of N-methyltyramine, a potent stimulant of gastrin release present in beer, after oral ingestion in rats by gastrointestinal-transit-absorption model. Drug Metab. Dispos., 28 (5) 577-581 (2000).　

C.W. Botka, T. W. Wittig, R. C. Graul, C. U. Nielsen, K. Higaki, G. L. Amidon and W. Sadee: Human proton/oligopeptide transporter (POT) genes: Identification of putative human genes using bioinformatics. AAPS J., 2 (2) issue 16 (2000).

K. Nakayama, H. Matsuura, M. Asai, K. Ogawara, K. Higaki and T. Kimura: Estimation of intradermal disposition kinetics of drugs (I): Analysis by compartment model with contralateral tissues. Pharm. Res., 16 (2) 302-308 (1999).

K. Ogawara, M. Yoshida, K. Higaki, T. Kimura, K. Shiraishi, M. Nishikawa, Y. Takakura and M. Hashida: Hepatic uptake of polystyrene microspheres in rats: effect of particle size on intrahepatic distribution, J. Controlled Release, 59 (1) 15-22 (1999).

Y. Oyama, H. Yamano, A. Ohkuma, K. Ogawara, K. Higaki and T. Kimura: Carrier-mediated transport systems for glucose in mucosal cells of human oral cavity. J. Pharm. Sci., 88 (8) 830-834 (1999).

K. Ogawara, M. Yoshida, J. Kubo, M. Nishikawa, Y. Takakura, M. Hashida, K. Higaki, and T. Kimura: Mechanisms of hepatic disposition of polystyrene microspheres in rats: Effects of serum depend on the sizes of microspheres. J. Controlled Release, 61 (3) 241-250 (1999).

K. Ogawara, M. Yoshida, Y. Takakura, M. Hashida, K. Higaki and T. Kimura: Interaction of polystyrene microspheres with liver cells: Roles of membrane receptors and serum proteins. Biochim. Biophys. Acta, 1472 (1-2) 165-172 (1999).

K. Ogawara, M. Yoshida, K. Furumoto, Y. Takakura, M. Hashida, K. Higaki and T. Kimura: Uptake by hepatocytes and biliary excretion of intravenously administered polystyrene microsphere in rats. J. Drug Targeting, 7 (3) 213-221 (1999).

K. Higaki, T. Yukawa, M. Takeuchi, K. Nezasa and M. Nakano: Stereoselective uptake of an organic anion across the renal basolateral membrane in isolated perfused rat kidney. Drug Metab. Dispos., 26 (2) 138-145 (1998).

Y. Emi, D. Tsunashima, K. Ogawara, K. Higaki and T. Kimura: Role of P-glycoprotein as a secretory mechanism in quinidine absorption from rat small intestine. J. Pharm. Sci., 87 (3) 295-299 (1998).

K. Nezasa, K. Higaki, M. Takeuchi, T. Yukawa and M. Nakano: Pharmacokinetics of a novel benzodiazepine partial inverse agonist in the F344 rat, SD rat and B6C3F1 mouse. Xenobiotica, 28 (5) 515-525 (1998).

S. Haruta, N. Iwasaki, K. Ogawara, K. Higaki and T. Kimura: Absorption behavior of orally administered drugs in rats treated with propantheline. J. Pharm. Sci., 87 (9) 1081-1085 (1998).

S. Yagi, K. Nakayama, Y. Kurosaki, K. Higaki and T. Kimura: Factors determining drug residence in skin during transdermal absorption. Biol. Pharm. Bull., 21 (11) 1195-1201 (1998).

K. Sugimoto, M. Yoshida, T. Yata, K. Higaki and T. Kimura: Evaluation of poly(vinyl alcohol)-gel spheres containing chitosan as dosage form to control gastrointestinal transit time of drugs. Biol. Pharm. Bull., 21 (11) 1202-1206 (1998).

T. Sawamoto, S. Haruta, Y. Kurosaki, K. Higaki and T. Kimura: Prediction of plasma concentration profile of orally administered drugs based on gastrointestinal transit kinetics and absorbability in rats. J. Pharm. Pharmacol., 49 (1) 450-457 (1997).

K. Higaki, Y. Matsumoto, R. Fujimoto, Y. Kurosaki and T. Kimura: Developmental changes in pharmacokinetics of recombinant human insulin-like growth factor-I in rats. Res. Commun. Mol. Pathol. Pharmacol., 97 (2) 115-124 (1997).

T. Kimura, Y. Murakawa, M. Ohno, S. Ohtani and K. Higaki: Gastrointestinal absorption of recombinant human insulin-like growth factor-I in rats. J. Pharmacol. Exp. Ther., 283 (2) 611-618 (1997).

K. Higaki, Y. Matsumoto, R. Fujimoto, Y. Kurosaki and T. Kimura: Pharmacokinetics of recombinant human insulin-like growth factor-I in diabetic rats. Drug Metab. Dispos., 25 (11) 1324-1327 (1997).

K. Higaki, M. Takeuchi and M. Nakano: Estimation and enhancement of in vitro corneal transport of S-1033, a novel antiglaucoma medication. Int. J. Pharm., 132 (1-2) 165-173 (1996).

K. Higaki: Stereoselective pharmacokinetics of a chiral organic anion in rats: analysis of its kinetics in liver and kidney. Ann. Rev. Shionogi Res. Lab., 46, 48-85 (1996)